Cryptobrachytone C from Cryptocarya pulchrinervia (Kosterm) Leaves on Proliferation, Apoptosis, Migration and Clonogenicity of MCF-7 and T47D Cell Lines

Cryptocarya pulchrinervia is an Indonesian indigenous plant that grows in Sumatra, Kalimantan and Papua. One of the new compounds extracted from this was cryptobrachytone C, which known to be cytotoxic against cancer cells Murine leukemia P388 with IC50 10.52 ?M. In study, cytotoxicity anticancer properties C on proliferation, apoptosis, migration clone formation MCF-7 T47D breast cell lines were examined, had not previously been done before. The compound measured using MTT (3- (4,5-dimethylthiazol-2- yl) -2,5-di-phenyl-tetrazolium bromide) assay. proliferation a BrdU assay, apoptosis annexin-V FITC, while transwell filter. test result demonstrated 12.94 ± 0.32 ?M but 65.33 2.33 nor normal MRC-5 122.57 19.84 assay showed at concentration antiproliferative (p < 0.05) did significantly reduce 0.07). Although results could induce cells, it insignificant > 0.05). concentrations inhibit cells. clonogenic can inhibition colonies. anti-cancer character more significant line compared T47D. This study potential as lines.